Determination of SPF in vitro

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Test ProceduresUV-Protection Factor

This method permits defined and standardised conditions for measurements, which are not influenced by biological factors such as pigmentation type, anti-inflammatory drugs, skin surface and individual technical variations (application, erythema, readings).In addition, the photo-stability of the sun protection substance can be determined.

In this context, the sunscreen is homogenously spread over a substrate and is then in accordance to the in vivo method irradiated using a solar simulator light source before the spectral transmittance is measured.

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